Osmosis Concentration Effects and Dialysis Tubing Dialysis

By examining the action of solutes such as potassium iodide and glucose, and weight loss changes, experimentation aimed at passive movement and how experimental moves the movement of the membranes.

This experiment investigates how much concentration of solutors influenced osmosis processes and inserting a semielmeable carming), checking the movement of water, changes in changes in the entire time.

Introduction

Osmosis and content are important cellular transport processes, which play important roles in maintaining homochemical functions (alberts ether to move into the biological molecules of water through a semipermermeable membrane from an area of ​​deeper Concentration of an area of ​​higher solut concentration until the balance is reached. On the contrast, the diffusion involves the action of soluting particles from an area of ​​lower concentration (binod, 2024).

Understanding the dynamics of these processes is necessary for exploring how cells associate their environment, absorb nutrients, and eliminate waste. There are three main types of solutions if osmosis: hypertonic, where there are many solutes outside the cell than within the cells Hypotonic, where there are fewer solutes outside the cell, which leads water to move to the cell; and isotonic, where the concentration of solutes is the same inside and outside the cell, resulting in any water movement (binod, 2024).

In this experiment, dialysis bags are used in the experiment as they are semermermetable and represent artificial cells to study osmosis and spray. Experiment hypothesis is that the starch / glucose solution within the dialysis tubing will show a change in color because of the spraying of potassium iodide. And the weight of the dialysis bags will change due to osmosis, depending on the concentration of sucrose.

Materials and Methods

Steps for Diffuse Study and Osmosis using Dialysis Tubing

Experimenting with Provision:

1. Prepare the diffusion solution:
   • Fill up a small water beaker.
   • Add 10 Three to Potassium iodide Solution to Beaker to achieve a medium brown color.
2. View of discoloration:
   • Record the initial color and shape of potassium iodide solution of beaker.
   • Wait for a solution to scatter, observe color change until the solution can be yellow altogether, indicating the perfect spray.
3. Prepare Dialysis Tubing:
   • Cut a piece of dialysis tubing that is approximately 10 cm in length.
   • Clakle is one end of the tubing safely and leave the other end open.
4. Fill in Dialysis Tubing:
   • Fill the open end dialysis tubing about two-thirds of a solution of starch / glucose.
   • Safe Clamp to open end of tubing.
5. Record initial observations:
   • Record the color of potassium iodide Solution to beaker and starch / glucose solution of dialysis tubing in Table 1.
   • Dip a glucose test strip into beaker solution and record the result of Table 2.
6. Put the dialysis tubing on Beaker:
   • Bind the prepared dialysis tubing of beaker with potassium iodide solution.
7. Wait and observe:
   • After 30 minutes, record changes in potassium iodide solutions beaker and starch / glucose solution within the dialysis tubing in Table 1.
   • Dip glucose test strip into beaker solution again and record the result of Table 2.

Experiment with osmosis:

After 60 minutes, record the final weights of all dialysis cells in Table 3.

Prepare osmosis solutions:

• Fill a small beaker about two-thirds full of a 25% Sucrose solution.
• Fill a large beaker about two-thirds full of a 1% Sucrose solution.

Prepare dialysis tubing for artificial cells:

• Get four pieces of soaked dialysis tubing.
• Clip at one end of each piece of dialysis tubing with a clip. Label the clips A, B, C, and D.

Fill in Dialysis Tubing:

• Open “Cell A” and fill it about two-thirds filled with a 1% Sucrose solution, then strong clamp.
• Fill in “Cell B” with 1% sucrose solution, “Cell C” with 10% sucrose solution, and “Cell D” with 25% Sucrose solution, stable clamping each other.

Record the first weights:

• Weigh each of the four dialysis cells and record their first weights in Table 3.

Wastes dialysis cells:

• Put “Cell A” on a small beaker with 25% Sucrose solution.
• Put “cells b, c, and D” on the big beaker with 1% Sucrose solution.

Wait and weigh:

• After 15 minutes, remove cells from their crooks, drying a little, and weigh it.
• Record the new weight on Table 3.
• Return the cells to their respective indigestions.

Repeat the process of weighing:

• Repeat the process to remove, dry, evaluate, and recording weight after 30 minutes, 45 minutes, and 60 minutes.

Last Weights:

• After 60 minutes, record the final weights of all dialysis cells in Table 3.

result

Experimental consequences appear to spray potassium iodide and glucose behavior within the dialysis tubing, as shown in the following tables.

Table 1. The Sprinkle of Potassium Iodine Solution from Beaker to Starch / Glucose solution from Dialysis Tubing

Table 2. Glucose Strip Test for spraying

Osmosis experimental results are shown in the action of the solute across the dialysis tubing and weight changes, indicating the weight, nature of nature.

Table 3. Change of weight in dialysis cells as a function of time

Discussion

Experiment shows that potassium iodide varies with the starch / glucose solution within the dialysis tubing, causing color change and confirmation of successful differences. In addition, the weight of dialysis bags is different based on their surrounding concentrations of Sucrose, which indicates the effects of osmosis hypertonic solutions that are lost.

The initial observations reveal that potassium iodide solution began as a yellowish brown, while the starch / glucose solution is white. After 30 minutes, Potassium iodide Solution

Becomes clear, and the starch / glucose solution has become purple / cloudy, indicating successful degradation in the tubing.

The beginning of glucose test was initially reflected in a teal color, indicating that there was no presence of glucose. At the end of the experiment, the brown / green strip has changed, confirm a trailing glucose to the beaker solution.

Weight changes in many hours show different answers to surrounding solutions. It shows a slight increase in the weight after 10 minutes but has been converted after indicating the hypertonic environment of beaker. Cell B remains relatively strong indicating an isotonic environment. On the contrary, the cell c shows a gradual reduction in weight, while cell d reveals a significant reduction, reflecting the water loss due to this hypertonic environment. Solute movement from higher concentration to low concentration to maintain homostasis (Alberts et al., 2014).

Experiment has weaknesses, like differences how good dialysis tubing is allowed to get through, which can lead to unfair consequences. Also, non-temperature control and dependent on color changes for measurements can be more reliable.

Overall, results support the hypothesis that differs from dialysis membrane and highlight the effects of osmotic pressure into the weighted dialysis bags.

Additional experiments

Incoming experiments can be reviewed how to affect osmosis temperature and spray, which expects higher temperatures make it easier to occur. We can also try different ingredients, such as salt or sugar, to see how they repeat the molecules.

References

Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2014). Molecular biology of cell (6th ed.). Science Science.

GC, Binod. “Osmosis and Variation: Differences and reasons to affect it.” The Science Notes14 Apr. 2023. Web. 2 October 2024. Osmosis and Difference: Differences and reasons affected by them

Mika, TA, Klein, RJ, Bullerjahn, AE, Connour, RL, Swimmer, LM, white,

Gosses, MW, Carter, Te, Andrews, Am, Good, JL, & Sidiq, F. (Ed.). (2024). Answer manual in Phymoly and Physiology BIO 211 (3rd ed.). Owens college community.

GC, Binod. “Cellular Transport: Passive and active mechanisms.” The Science Notes3 September 2024. Web. 2 October 2024. Cellular Transport: Passive and Active Mechanism – Science Notes