Getting Hemolymph by Cardiac Puncture: Grammarero spp research tool. (Araneae: Thraphosidae)
Abstract
This study focuses on collecting and analyzing hemolymph from adult spiders belonging to the genus Grammosola, specifically for those traded as G. Porteri. Using cardiac puncture procedure, hemolymph is obtained with no significant damage to arachnids, representing an ethical choice of traditional procedures in need of limiting extraction. Morphological results confirm that specimens show typical genus grammatical traits. Hemolymph’s protein components are analyzed, showing a participation in the bands of 70 and 25 Kda. Hemocytes to healing primary grans, and phylogenetic analysis allowed to group different families and genera within the suborders Maligomorphae. In addition, the implications of G. Porteri’s taxonomy are referred to, discussing its classification as a synonym of Mr. Rosea. This study not only causes the understanding of the variant and systems in Chile tarantulas but also suggested a new research research method.
