Cvill6 and cvill7: trapentive and selective peptide blockers in the kv1.2 ion channel detached from Mexican scorpion centruroides villlegasi
Abstract
Scorpion Venoms is a rich source of peptides to grow ion channels activity and can serve as a new medication for Channelopathathiya. CVILL6 and CVILL7 two new peptes that have been detached from the poison of CENTURUROIDES your wildlife With MW 4277 DA and 4287 DA and they consist of 38 and 39 amino acids, in fact, including six cysteines. Aligning a series of alignment revealed the high-sized α-ktx2 members subfamily in potassium channel toxins. In the electrophysiology, Cvill7 strongly holds the KV1.2 ion channels with an IC50 at 16 PM and kv1.3 with IC50 at 7.2 nm. Besides, it shows partial activity in KCA3.1 and KV1.1, with ~ 16% and ~ 34% restrictions in 100 NM, respectively. On the contrary, Cvill6 is blocked by Kv1.2 with low ability (IC50 In 3.9 nm) and shows moderate prohibition of Kv1.3 (~ 11%) and KCA3.1 (~ 27%) in 100 nm concentration. Neither peptide shows any activity against other k+ Tests the canals in this study (Kv1.5, KV11.1, KCA1.1, and KCA2.2). Especially, cvill7 has a remarkable association of kv1.2 and high choice of 450-fold in kv1.3 and 12,000-fold in kv1.1. These pharmacological properties make Cvill7 a potential candidate to target kv1.2 profit function (Gof) -Rinated Channelopathies.
