By Rosa Pineda – Own Jobs, CC BY-SA 3.0, https: //commons.wikimedia.pord/index.phpurid=27718135
Inhibitory potential of three scorpion venom peptides against multidrug-resistant to klemoyella pnemoniae
History:
The increase in bacterial infections that prevent drugs, especially the cause of Klebsliella pneumoniae promoted urgent need for therapeutic novel candidates. Infections obtained at the hospital from K. Pneumoniae Carbapeemase (KPC) -producing bacteria has a serious threat to health, especially the impact of respiratory and urine tracts. This study investigates the effectiveness of antibacterial in three antimicrobial peptides, called Pantinins, from Emperor of the Pandinus Soldom ‘Venom against K. Pneumoniae and various clinical production clinical KPC.
Ways:
Non-poisonous concentrations were established for each peptide using MTT method. Antibacter activity produced by liquid microdilutution assay to determine the minimum inhibitory (mic) concentration and the minimal octericidal (MBC). A test at the time held was made by recording colonies forming units (CFUS) several times and compared to unstructured bacteria. Finally, the peptese effect of declaring the gene of virulan reasons to K. Pneumoniae Checked by QPCR.
Results:
Antibacterial assays show the bactericidal effects for Pantinin-1 and Pantinin-2 in 1 h, while Pantinin-3 showed bacteriostatic properties. Have suitability, mic values from 6 to 25 μm for K. Pneumoniae and from 25 to 50 μm for KPC strains. Pantinins are setting their antimicrobial effect through a membragytic mechanism, directly associated with outer membrane lipopolysaccharides (LPS) spectopy (sem). Besides, QPCR shows a decrease in the Virlap gentleman stated, suggesting the effect of antibacterial peptides at the transcriptional level.
Conclusion:
These findings support pantinins as promised multidrug-resistant treatment candidates K. Pneumoniae infection.
Giugliano, R., Della Maria, R. The Inhitory Potential of Three Scorpion Venom Postides against Multidrug-resistant to Klebsontiella Pnemoniae. Fronties in Microbiology,, 161569719. https://doi.org/10.3389/fmicb.2025.1569719
