Recognizing and validating simple sequence SCeathats (SSR) markers for Androctoronus Crassicauda Scorpion using data-seq data in RNA-SEQ
Abstract
Objective
Simple Sequence Repetition (SSR) markers or microsatellites are one of the best and most complete molecular tools due to appropriate genomic coverage and high repetition. The simple sequences of markers for Scorpion Andrakonus Crassicoudoududa have not been reported so far. Therefore, this study is made with the intention of identifying and validating simple repositions of signs of sequence scorpoudauda using RNA-SEQ data.
Materials and Methods
Samples were assessed by qualified and the amount after retrieving RNA from Scorpion Venom Gland. Then the samples followed the Illamina Hyeq 2000 platform. The reconstruction of the transcripts made by De Novo Assembly by using Trinity Software. Next, to identify new SSR markers, Scorpion andRachtonus Crassicoudoududa transcriptore is analyzed using FullSR software (version 1.5). To validate the SSR markers, 8 pairs of primers are designed by Primer 3 software and checked by polymerase chain reaction through polymerase tissue obtained from scorpion tissue.
result
Preduction predicts through Trinity Program make a total of 744,804 transcripts and 563,526 unnenes. In this study, the 952,725 examination of FullSR software sequences lead to the Identification of 315,395 SSR markers. Among SSRS, two and three gluceotides are deemed for the highest number of repetitions with 71.85% and 22.36%, in fact. Among the 8 studied loci, only 2 shown in polymorphism. The expected and observed hetero is the fixation index statistic (FIS) for two loci nearly 0.1, which indicates low population pours. Besides, Chi-square sprute shows that the population is not at stet-werberg bacilibrium for Loci.
Conclusion
Different genetic genetical behaviors do all indicate that the population is more varied, but some factors reduce differentiation among the studied populations. This means that the population does not have a balance and the number of observed heterozygotes is less than expected heterozygotes. It is necessary to check the reasons that bear reduction in diversity to prevent further reduction in it. Overall, the consequences of this research shows that new SSRs can be useful for understanding the structure of the population and investigation of scorpion andrakonus crassicoudoududa.
