Identifying and designing an analgesic opioid cyclic peptide from Defensin 4 in Mebobhus Martensii Karsch Scorpion Venom more effective than morphine

Considering the limits of use and effects of analgesics, the new analgesics discovery should be discovered. Venoms of organisms are an important source of analgesic peptides. In this study, using computional comparisons such as molecular simulation, molecular dynamics, a non-poisonous machine-based and biological 9-amino acid algorithms from Defensin 4 to Merbourhus Marketsi Karsch Scorpion Venom was discovered for the first time and named Buchicyclin. Peptide is marked cyclically cycle by creating a disulfide bond, and its second structure is determined by the circulation Dichroism (CD). Next, the toxicity of the peptide is assessed using MTT, hemolysis, and deadly doses (ld50) Methods. Then, in animal tests using the tail-flick, hot plate, and methods formed by paw-lick the buntingcliin has a coil-like structure and can be considered a cyclic coil with disulfide bond. This peptide is not poisonous is ld50 In this peptide is higher than 20 mg / kg. Ang tanan nga mga pagsulay sa analgesic nagpakita nga ang Buthiusicclin peptide, sa 1 mg / kg / 2 mg / kg (kg / kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg (kg Gipakita usab sa mga resulta nga ang Buthiyclin mahimo nga mogamit sa Analgesic effect by binding opioid receptors. Given the loud and lasting analgesic effects of buthiusicclin and it cannot be considered a promising annalesic drug. However, this claim requires more clinical and clinical trials.